8 BIOTECHNOLOGY (UHS)

8 BIOTECHNOLOGY (UHS)

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Question 1 of 28

1. Question
Q.1 Prosomes are used in gene therapy against
- A) Hypercholesterolemia
- B) Coronary Artery Angioplasty
- C) Cystic Fibrosis
- D) Severe Combined Immunodeficiency Syndrome (SCID)
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Question 2 of 28

2. Question
Q.2 Genetically engineered cells are introduced into bone marrow cells in the treatment of
- A) Hypercholesterolemia
- B) Severe Combined Immunodeficiency Syndrome
- C) Cystic Fibrosis
- D) Coronary Artery Angioplasty
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Question 3 of 28

3. Question
Q.3 The common vectors used in recombinant DNA technology are
- A) Probes
- B) Palindromes
- C) Plasmids
- D) Prions
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Question 4 of 28

4. Question
Q.4 The enzyme used to isolate gene from DNA is
- A) Helicase
- B) Reverse Transcriptase
- C) Restriction Enzyme
- D) DNA Polymerase
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Question 5 of 28

5. Question
Q.5 Which one of the following enzymes is temperature insensitive?
- A) DNA Polymerase I
- B) Taq Polymerase
- C) DNA Polymerase III
- D) RNA Polymerase
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Question 6 of 28

6. Question
Q.6 In recombinant DNA technology _________ are tools for manipulating DNA
- A) Viruses
- B) Chromosomes
- C) Enzymes
- D) Genes
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Question 7 of 28

7. Question
Q.7 In DNA finger printing process, the use of __________ produces distinctive pattern on autoradiography or X-ray film
- A) Restriction enzyme
- B) Microsatellites
- C) Macrosatellites
- D) Probes for genetic markers
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Question 8 of 28

8. Question
Q.8 In the recombinant DNA technology plasmids are used as
- A) Genetic material
- B) Enzymes
- C) Vectors
- D) Probes
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Question 9 of 28

9. Question
Q.9 In which process, multiple copies of the desired genes are produced?
- A) Polymerase chain reaction
- B) Gene sequencing
- C) Analyzing DNA
- D) DNA finger printing
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Question 10 of 28

10. Question
Q.10 The enzyme adenosine deaminase is missing in person suffering from:
- A) Cystic fibrosis
- B) Hypercholesterolemia
- C) Severe combined immunodeficiency syndrome
- D) Parkinson’s disease
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Question 11 of 28

11. Question
Q.11 The DNA molecule formed from messenger-RNA by reverse transcriptase is called??
- A) Complementary DNA
- B) Recombinant DNA
- C) Chimeric DNA
- D) Plasmid DNA
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Question 12 of 28

12. Question
Q.12 The agent which separates the two strands of DNA in PCR is??
- A) DNA ligase
- B) Primer
- C) Heat
- D) Helicase
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Question 13 of 28

13. Question
Q.13 Cystic fibrosis patient lack a gene that codes for trans-membrane carrier of??
- A) Na+ ions
- B) Cl― ions
- C) Ca++ ions
- D) K+ ions
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Question 14 of 28

14. Question
Q.14 The phage commonly used as a vector in genetic engineering is?
- A) Lambda phage
- B) Gamma phage
- C) T2 phage
- D) T4 phage
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Question 15 of 28

15. Question
Q.15 Restriction endonucleases are naturally occurring enzymes of:
- A) Viruses
- B) Bacteria
- C) Fungi
- D) Plants
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Question 16 of 28

16. Question
Q.16 pBr 322 have antibiotic resistance gene for
- A) Ampicillin and aspirin
- B) Streptomycin and metronidazole
- C) Ampicillin and Tetracycline
- D) Penicillin and metronidazole
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Question 17 of 28

17. Question
Q.17 Cystic Fibrosis affects which one of the following cells of the body?
- A) Epithelial cells
- B) Endothelial cells
- C) Plasma cells
- D) Blood cells
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Question 18 of 28

18. Question
Q.18 The enzymes which act as molecular scissors in recombinant DNA technology is
- A) Exonucleoses
- B) Endonucleoses
- C) Polymerases
- D) Reverse transcriptases
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Question 19 of 28

19. Question
Q.19 Which of the following is the correct sequence of PCR?
- A) Heating Cooling Add Primer Copying of strand
- B) Heating Add Primer Cooling Copying of strand
- C) Add Primer Heating Cooling Copying of strand
- D) Cooling Add Primer Heating Copying of strand
Correct

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Question 20 of 28

20. Question
Q.20 When two different pieces of DNA are joined together, the result is which one of the following?
- A) Complementary DNA
- B) Mutated DNA
- C) Recombinant DNA
- D) Cloned DNA
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Question 21 of 28

21. Question
Q.21 In cystic fibrosis, liposomes-microscopic vesicles are sued which are coated with:
- A) Healthy Gene
- B) Chromosome
- C) Protein
- D) Carbohydrate
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Question 22 of 28

22. Question
Q.22 The DNA formed by the reverse transcription is called:
- A) rDNA
- B) dDNA
- C) cDNA
- D) DNA
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Question 23 of 28

23. Question
Q.23 Bacterial cells take up recombinant plasmids when they are treated with:
- A) CaCl2
- B) NaCl
- C) KCl
- D) NaOH
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Question 24 of 28

24. Question
Q.24 Which one of the following is made up of radioactively labelled nucleotides?
- A) Phage DNA
- B) Genomic Library
- C) Recombinant DNA
- D) Gene Probe
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Question 25 of 28

25. Question
Q.25 A technique in transgenic animals in which desired gene is inserted into the eggs of animal is
called:
- A) Embryonic Stem Cell mediated Transfer
- B) Microinjection
- C) Retro-virus mediated gene Transfer
- D) Virus vectors
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Question 26 of 28

26. Question
Q.26 The modified plasmid or phage DNA is called:
- A) Clone DNA
- B) Recombinant DNA
- C) cDNA
- D) rDNA
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Question 27 of 28

27. Question
Q.27 Restriction endonucleases cleave the __________ of duplex DNA.
- A) Nitrogenous base
- B) Base sugar
- C) Phosphodiester bond
- D) Hydrogen bond
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Question 28 of 28

28. Question
Q.28 The enzyme which is responsible for the formation of bond between two double stranded DNA
fragments is:
- A) Endonuclease
- B) Urease
- C) Ligase
- D) Helicase
Correct

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